因(yin)为密(mi)封套圈尖端是一(yi)样的，差别制作商的色谱(pu)柱(zhu)柱(zhu)头罗纹深(shen)度(du)不分(fen)歧，能(neng)够(gou)会呈现拧紧后色谱(pu)柱(zhu)端面没法与PEEK垫片齐平，但不影响利用。
栓接流程
将(jiang)在线过(guo)滤器直连上色(se)谱柱
1.国际规(gui)范色谱柱毗连
手动拧紧在线过滤器后(hou)请再利(li)用(yong)扳手旋(xuan)拧30°。
2.非规范色谱柱(zhu)毗连(lian)
较深罗纹柱头色谱柱可将PEEK垫片取上去手动拧紧后再利用扳手旋拧15°。
*如发明将PEEK垫片取下后仍然没(mei)法零死体积毗连，则产物与色(se)谱(pu)柱(zhu)(zhu)柱(zhu)(zhu)头罗纹不婚配。
注重(zhong)：请谨慎(shen)装置，不(bu)要过分旋拧(ning)在线过滤(lv)器(qi)，金属(shu)密(mi)封套圈(quan)发生的扭力能够会破(po)坏(huai)色谱柱(zhu)柱(zhu)头罗纹。
Designed for NanoLC, Not Scaled-Down HPLC
At nanoflow rates, even a small internal volume can have a measurable impact on chromatographic
efficiency. This kind of Guard Filter is engineered around the requirements of modern nanoLC systems:
1. Low internal volume as 10 nL to minimize extra-column dispersion
2. Precision filtration to prevent particulate contamination
3. Optimized flow path for stable nanoflow operation
4. High-pressure construction up to 1,200 bar for nano UHPLC applications
5. Bioinert porosity filter for improved sample recovery
6. Zero dead-volume to build in Valve port and Column
Built for LC-MS and Proteomics
The filter is particularly suitable for applications where chromatographic efficiency and peak capacity are
critical, such as Single-cell proteomics and Spatial proteomics. When routinely run these samples, filter is
much better for sample recovery than a trap column. If you are an intact protein researcher, detergents are
widely used during protein extraction, solubilization, membrane-protein preparation, and other sample
processing steps. Detergent-containing contaminants interception is inevitable for downstream nano LC
MS analysis.
滤片改换
• 旋开在线过滤器底座，改换滤芯，滤芯片装置无标的目的请求。
• 改换竣事后手动拧紧底座，再利用扳手旋拧180°使得(de)底座圆环轻(qing)触滤片塑(su)料环完成密封(feng)。
